NDRG1 is a member of the emerging NDRG family involved in stress responses, hormone responses, cell growth, and differentiation.
The NDRG1 gene is mapped to human chromosome 8q24.2 and encodes a cytosolic protein containing three unique tandem repeats of 10 hydrophilic aa near the C-terminus.
Expression of NDRG1 is markedly upregulated in various cells under such conditions as cell differentiation, hypoxia and exposure to heavy metals.
However, its expression is repressed by the Myc family transcription factors and after cellular transformation.
Mutations in the NDRG1 gene are linked to the hereditary motor and sensory neuropathy.
The protein is ubiquitously expressed in human and rat heart, brain, kidney, placenta, lung, liver, skeletal muscle, and pancreas.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.1-0.3ug/ml, observed in lysates of human brain, kidney and testis on ~48kD bands
Immunohistochemistry (Paraffin): 2-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human NDRG1. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to GNSAGPKSMEVSC, from human NDRG1, at C-terminal (NP_006087.2, NP_001128714.1).
