Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1).
It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates.
May also be involved in pre-mRNA splicing.
Binds DNA and might act as a transcriptional repressor.
Seems to be required for cell proliferation.
Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions.
It generates 5'-phosphate termini at the site of cleavage.
This isoform does not inhibit PP-1.
May be implicated in mRNA splicing.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Immunohistochemistry (Paraffin): 2.5-3.8ug/mlWestern Blot: 1-2ug/ml, observed in human lung and H460 cell extracts on ~40kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human PPP1R8. Species sequence homology: Bovine, canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-EAWPGKKPTPSLLI, from human PPP1R8, at C-terminal (NP_002704.1, NP_054829.2, NP_612568.1).
