Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins.
It has a pro-survival role in the early stages of apoptosis, and is thought to prevent cytochrome c release from mitochondria.
Mcl-1 is also essential for embryogenesis and for the development and maintenance of B and T lymphocytes.
Mcl-1 is a highly regulated protein induced by a wide range of survival signals, including cytokines and growth factors.
During apoptosis, Mcl-1 is rapidly degraded by the proteasome pathway, and is also readily cleaved by caspases to produce a potent cell death promoting protein.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000Western Blot: 1-3ug/ml, observed in lysates of human lung, in mouse and rat thymus, and in cell line K562 on ~35kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human MCL1. Species Crossreactivity: Mouse and rat. Species sequence homology: Bovine, canine ad porcine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-AGVGAGLAYLIR, from MCL1, at C-terminal (NP_068779.1, NP_001184249.1).
