Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides.
Binds hemi-methylated DNA as well.
Recruits histone deacetylases and DNA methyltransferases.
Acts as transcriptional repressor and plays a role in gene silencing.
Isoform 1 may enhance the activation of some unmethylated cAMP-responsive promoters.
Reports about DNA demethylase activity of isoform 2 are contradictory.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.3-1ug/ml, observed in lysates of cell line Jurkat on ~48-50kD bandsImmunohistochemistry (Paraffin): 2-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human MBD2. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-RNDPLNQNKGKPDLN, from human MBD2, at the internal region of the protein (NP_003918.1).
