Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis.
Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
MMP12 may be involved in tissue injury and remodeling.
This protein has significant elastolytic activity.
MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine.
Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3.
The protein is found in alveolar macrophages but not in peripheral blood monocytes.
MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Western Blot: 0.3-1ug/ml, observed in human breast cancer lysates on ~55kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human MMP12. Species sequence homology: Bovine and canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu
Immunogen：Synthetic peptide corresponding to C-RPKTSVNLISS, from human MMP12, at the internal region of the protein (NP_002417.2).
