Acts as a transcriptional coactivator for NOTCH proteins.
Has been shown to amplify NOTCH-induced transcription of HES1.
Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8.
Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription.
Induces phosphorylation and localization of CREBBP to nuclear foci.
Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Immunohistochemistry (Paraffin): 2.5-3.8ug/mlWestern Blot: 0.1-0.3ug/ml, observed in human skeletal muscle lysates on ~110kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human MAML1. Species sequence homology: Mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to VLPTCPMAEFALPR, from human MAML1, at N-terminal (NP_055572.1).
