Leukocyte-specific protein (LSP; also known as Lymphocyte-specific protein1, SP34 and pp52) is a 52kd filamentous-actin-binding intracellular phosphoprotein.
LSP is localized to the internal surface of the plasma membrane, cytoplasm, and actin filaments.
LSP expressed in various eukaryotic cell lines such as the melanoma cell line A7 but not in other hematopoietic lineages.
Studies showed that LSP play a key role in anti-IgM-induced apoptosis of the B-cell lymphoma line as well as cytoskeleton-driven regulation.
LSP contains a high-affinity F-actin binding site, which recently is identified in the basic C-terminal half.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.05-0.2ug/ml, observed in human lymph node, tonsil and peripheral blood mononucleocytes lysates on ~50kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human LSP1. Species Crossreactivity: Mouse and rat. Species sequence homology: Bovine and canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-RYKFVATGHGKYEKV, from LSP1, at C-terminal (NP_002330.1, NP_001013271.1).
