Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80kD.
The protein has two iron binding domains each housing one Fe 3+ and the synergistic CO 3 2- ion.
The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO 32- and 98 carbohydrate atoms.
Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei.
Intravenous infusion of lactoferrin is protective against lethal doses of E.
coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion.
Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia.
Lactoferrin has been shown to have a role in the immune system and in early development of the embryo.
A specific receptor for lactoferrin binding has been implicated in the human fetal intestine.
Early embryonic localization of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.05-0.2ug/ml, observed in human peripheral mononucleocyte lysates on ~85kD bandsImmunohistochemistry (Paraffin): 2-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human LTF.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-ENYKSQQSSDPDP, from human LTF, at the internal region of the protein (NP_002334.2).
