Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes.
LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+.
It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate.
In mammals, three types of LDH subunits (35kD) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C.
All LDH subunits can combine to form various terameric isoenzymes (140kD).
LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA (peptide): 1:16,000Western Blot: 0.03-0.1ug/ml, observed in mouse testis lysates on ~30-35kD bandsImmunohistochemistry: Seminiferous tubules of mouse testisOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes mouse LDHC.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to C-KLGTDSDKEHWKNIH, from mouse LDHC, at the Internal region of the protein (NP_002292.1, NP_059144.1). Species sequence homology: Human
