84-1225-57　IDH1 (Isocitrate Dehydrogenase [NADP] Cytoplasmic, IDH, Cytosolic NADP-isocitrate Dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) 100ug　I8850-40C

特徴

• IDH1 belongs to two distinct subclasses.
• The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.
• This protein contains the PTS-1 peroxisomal targeting signal sequence.
• The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.
• The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000Western Blot: 0.1-0.3ug/ml, lysates of cell line HepG2 observed on ~40-45kD bandsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.