Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 2.5mg/ml, on stain rat cortical neurons ELISA: 1:16,000Western Blot: 0.3-1ug/ml, observed in lysates of cell line HEK293 on ~70-75kD bandsImmunohistochemistry (Paraffin): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes human IFT74. Species Crossreactivity: Rat.
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Rt
Immunogen:Synthetic peptide corresponding to C-KTIVDALHSTSGN, from IFT74, at C-terminal (NP_001092692.1, NP_001092693.1).