Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Binds to sites in the 5'-flanking sequence of its coding region with various affinities.
The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.1-0.3ug/ml, human colon lysates observed on ~36kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes human HOXA4. Species sequence homology: Canine.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ca Hu
Immunogen:Synthetic peptide corresponding to C-QAKGPAHGLHASH, from human HOXA4, at the internal region of the protein (NP_002132.3).