HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif.
It is highly and ubiquitously expressed during embryogenesis.
Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively.
Human and mouse HMGA2 share 95% aa sequence identity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 1-3ug/ml, observed in human heart lysates on ~18kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human HMGA2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KAAQKKAEATGEK, from human HMGA2, at the internal region of the protein (NP_003474.1, NP_003475.1).
