Human glutamate receptor 4 (GRIA4) is a new member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain.
Binding studies showed that human GRIA4 transfected into simian kidney cells (COS-1) exhibits high specific binding for[3H](RS)-alpha-amino- 3-hydroxy-5-methylisoxazole-4-propionic acid ([3H]AMPA) but not [3H]kainate.
Ion substitution experiments indicate that hGluR4 receptor-linked ion channels in their homomeric state are permeable to both CA2+ and Na+ ions.
Immunoprecipitation studies suggest that GRIA1 exists in situ in the form of a pentamer.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.3-1ug/ml, observed in human brain (cerebellum) lysates on ~90kD bandsImmunohistochemistry (Paraffin): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human GRIA4. Species sequence homology: Bovine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-KKLDQREYPGSETP, from human GRIA4, at the internal region of the protein (NP_000820.3, NP_001070711.1, NP_001070712.1).
