Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development.
The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.
A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity.
In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation.
Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes GLI3. Species sequence homology: Canine, human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-HNKRSKIKPDED, from GLI3, at the internal region of the protein (NP_000159.2).
