Gelsolin is a calcium regulated actin binding protein.
Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6).
When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity.
Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites.
Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml, observed in human spleen lysates on ~90kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human GSN. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-PRLKDKKMDAHP, from human GSN, at the internal region of the protein (NP_000168.1, NP_937895.1).
