Gross cystic disease of the breast is benign premenopausal disorder in which cysts are a predominant pathological lesion.
These cysts appear to be formed from excessive aporcine cystic secretions.
This fluid is composed of several glycoproteins including a unique 15kD monomer protein, PIP.
Cytosolic analysis of normal tissue specimens from all major organs has demonstrated PIP in aporcine epithelia, lacrimal, ceruminous and Mollus glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands.
PIP and prostate specific antigen are co-expressed in androgen receptor-positive breast tumors.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.3-1ug/ml, observed in lysates of cell line A431on ~14kD bandsImmunohistochemistry (Paraffin): 2-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human PIP.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-GANKAQDNTRK, from human PIP, at the internal region of the protein (NP_002643.1).
