MID2 is a member of the tripartite motif (TRIM) family.
The MID motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
The protein localizes to microtubular structures in the cytoplasm.
Its function has not been identified.
Alternate splicing of the gene encoding MID2 results in two transcript variants encoding different isoforms.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 1-3ug/ml, observed in mouse heart lysates on ~80kD bandsImmunohistochemistry (Paraffin): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human MID2. Species Crossreactivity: mouse. Species sequence homology: Bovine, canine, porcine nd rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to QESPYVSGMKTCH, from MID2, at C-terminal (NP_036348.2, NP_438112.2).
