FoxA1 is a 473aa, 49kD (predicted) protein of the forkhead nuclear transcription factor family.
It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors.
FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines.
Within the sequence used as an immunogen, human FoxA1 shares 93-86aa identity with mouse and rat FoxA1, respectively.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.2-0.6ug/ml, observed in human lysates of cell line HepG2 on ~50kD bandsImmunohistochemistry (Paraffin): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human FOXA1. Species Crossreactivity: mouse. Species sequence homology: Bovine, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-GVYSRPVLNTS, from FOXA1, at C-terminal (NP_004487.2).
