Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression.
In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression.
RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs.
Associated with polysomes.
May play a role in the transport of mRNA from the nucleus to the cytoplasm.
Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Peptide): 1:128,000Western Blot: 0.1-0.3ug/ml, observed bands in lysates of cell line KELLY at ~80kDOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA 0.02% sodium azide.
Specificity：Recognizes human FMR1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-RTGKDRNQKKEKPD, at the internal region near the C-terminal of human FMR1 (NP_002015.1). Species Sequence Homology: canine, mouse and rat.
