FBP17, a 71kD microtubule binding protein, is a member of Formin binding protein family.
It consists of a CIP4 homology (FCH) domain at the N-terminus, followed by first coiled-coil domain, a proline rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBP) and a C-terminal SH3 domain.
FBP17 binds to dynamin with its FCH domain and induces tubular membrane invaginations thereby participating in dynamin-mediated endocytosis.
It also interacts with Rho family GTPases and Snx2, a protein involved in EGFR signaling and plays a significant role during cytoskeleton organization, cell polarity and signal transduction.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.25-2ug/ml, observed in human testis lysates on ~70kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human FNBP1. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to KQLESSKRRFERDC, from human FNBP1, at the internal region of the protein (NP_055848.1).
