Fibulin 5, also known as DANCE and EVEC, is a secreted 55kD matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis.
Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44aa spacer region, five more tandem EGF-like domains, and a 115aa Fibulin-like C-terminal region.
Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5.
Fibulin 5 i s expressed by smooth muscle cells and endothelial cells of the developing asculature as well as by migrating neural crest cells and l unginterstitial fibroblasts.
It is downregulated in the adult vasculature but is re-expressed at aortic branching points, in the uterus, and at sites of mechanical or at herosclerotic injury.
The RGD motif of Fibulin 5 binds to several cell surface Integrins including aVb3, aVb5, a9b1, a4b1, and a5b1.
The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix.
Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2, and the crosslinking lysyl oxidase-like enzymes LOXL1, 2, and 4 along Fibrillin microfibrils.
In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevent s Fibulin 5 from interacting with Fibrillin-1 microfibrils.
Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation a migration and by limiting vascular sprouting.
Fibulin 5 is downregulated in many malignancies.
When experimentally re-expressed, however, Fibulin 5 enhances tumor cell invasiveness and cooperates with TGF-b i n initiating and promoting epithelial-mesenchymal transition (EMT).
Defects in Fibulin 5 expression or function can result in a loss of connective tissue Integrity, cardiac elasticity, and ability to remodel the vasculature after injury.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.1-0.3ug/ml, observed in human colon, heart and ovary lysates on ~50kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human FBLN5. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-RPIKGPREIQLDLE, from human FBLN5, at the internal region (near the C-terminal) of the protein (NP_006320.2).
