FHL1 is a member of the four-and-a-half-LIM-only protein family.
Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger.
Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.05-0.3ug/ml, observed in human muscle lysates on ~30kD bandsImmunohistochemistry (Paraffin): 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human FHL1. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to CNKRFVFHNEQVY, from FHL1, at the internal region of the protein (NP_001440.1, NP_001153171.1).
