Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 0.3-1ug/ml, heart and kidney lysates from mouse and rat observed on ~75kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes FEM1C. Species Crossreactivity: Mouse and rat. Species sequence homology: Bovine, canine and human.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Bo Ca Hu Mo Rt
Immunogen:Synthetic peptide corresponding to C-HIPEKLETFVSLHR, from FEM1C, at C-terminal (NP_064562.1).