F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains.
Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins.
FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 2-6ug/ml, observed in human brain (cerebellum) lysate on ~25kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human FBXO44.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-AALTPPEPPSAEP, from human FBXO44, at C-terminal (NP_904319.1).
