Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation.
Preferentially uses arachidonate and eicosapentaenoate as substrates.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml, observed in lysates of cell line HepG2 on ~80kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes human ACSL4. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Bo Ca Hu Mo Po Rt
Immunogen:Synthetic peptide corresponding to C-HYLKDIERMYGGK, from human ACSL4, at C-terminal (NP_004449.1, NP_075266.1).