Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.
Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.
Plays an important role in angiogenesis and tumor neovascularization.
The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2.
Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation.
Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK.
Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.01-0.03ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes EFNA1. Species Crossreactivity: Mouse and rat. Species sequence homology: Canine, porcine and human.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-KVTVSGKITHSP, from EFNA1, at the internal region of the protein (NP_004419.2).
