EN2 is thought to have a role in controlling development.
In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments.
Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal.
This protein has been implicated in the control of pattern formation during development of the central nervous system.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:4000Western Blot: 0.1-1ug/ml, observed in mouse and rat brain lysates on ~30kD bands.
Primary incubation 1 hour at room temperature
Immunofluorescence: 10ug/ml.
Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes rat EN2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to C-NHSTTAKEGKSDSE, from rat EN2 at C-terminal (NP_001418.2). Species sequence homology: Canine and human
