ELMO1 protein, initially identified as a mammalian homologue of C.
elegans CED-12, is required for cell migration and engulfment of dying cells.
The human ELMO1 gene is localized on chromosome 7p14.2 and encodes a polypeptide with 44% similarity to C.
elegans CED-12.
Multiple transcript variants encoding three different isoforms for the protein has been identified.
ELMO1 is a soluble cytoplasmic protein with a potential SH3 binding PxxP motif near the C terminus (conserved from worm to human), a putative leucine zipper around aa630 (conserved in all mammalian proteins) and a putative pleckstrin homology (PH) domain.
It is widely expressed in all tissues.
The protein functionally cooperates with Crk and dedicator of cytokinesis 1 (Dock180), and promotes phagocytosis and morphological changes.
Applications:Suitable for use in ELISA, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 1-2ug/ml, Jurkat cell lysate observed on ~50, 70kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human ELMO1. Species Crossreactivity: Bovine and zebrafish. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Bo Ca Hu Mo Rt Ze
Immunogen：Synthetic peptide corresponding to PKEPSNYDFVYDCN, from ELMO1, at C-terminal (NP_055615.8, NP_569709.1, NP_001034548.1).
