The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators.
The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases.
In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver.
Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 2.5ug/ml, in human U87-MG glioblastoma lysate on ~100kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human ECT2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to AENSVLTSTTGRT-C, from human ECT2, at N-terminal (NP_060568.3).
