DUSP16 is a 665aa novel mitogen-activated protein kinase phosphatase belonging to the non-receptor class of the protein-tyrosine phosphatase family with a rhodanese-like domain, protein tyrosine phosphatase catalytic domain and a long terminal stretch containing nuclear export and localization signal.
DUSP16 are dual-specificity phosphatases that dephosphorylate the TXY motif of MAPK TXY motifs, thereby inactivating MAP kinases.
DUSP16 is ubiquitously expressed and behaves both as a nuclear shuttle and as a phosphatase.
It binds to and inactivates p38 MAPK and JNK/SAPK, p38 alpha and -beta but not gamma or delta and ERK.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Immunohistochemistry (Paraffin): 2-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human DUSP16. Species sequence homology: Bovine, canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to AHEMIGTQIVTER-C, from human DUSP16, at N-terminal (NP_085143.1).
