Ferric-chelate reductase that reduces Fe3+ to Fe2+.
Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe3+ thereby facilitating its transport into the mucosal cells.
Uses ascorbate as electron donor.
May be involved in extracellular ascorbate recycling in erythrocyte membranes.
May also act as a ferrireductase in airway epithelial cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA (Peptide): 1:16,000Western Blot: 0.1ug/ml using human colon lystates showed bands at 35kD, 26kD and 28kD
Immunohistochemistry (Paraffin): 2-5ug/ml using human testis shows membranous staining in primarily myofibroblasts of the basement membrane surrrounding the seminiferous tubules
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Specificity：Recognizes human CYBRD1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to CRNLALDEAGQRSTM at the C-terminal, from human CYBRD1 (NP_079119.3). Species Sequence Homology: bovine, canine and porcine
