The Dscam family of cell surface proteins mediates recognition events between neurons that play an essential role in the establishment of neural circuits in many organisms.
Interneurons form synaptic connections with retinal ganglion cells in specific layers.
Dscam and DscamL are expressed in different subsets of interneurons and ganglion cells.
Interestingly, interneurons and ganglion cells that target to the same layer, express the same Dscam protein.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 2-6ug/ml, human brain, cerebellum lysates on ~170kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes human DSCAM. Species sequence homology: Canine, mouse and rat.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ca Hu Mo Rt
Immunogen:Synthetic peptide corresponding to C-DSWDSAQRTKDVSPQ, from human DSCAM, at the internal region of the protein (NP_001380.2, NP_996770.1).