Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER).
Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins.
Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase.
Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.
Applications:Suitable for use in Immunohistochemistry, Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:32,000Western Blot: 0.1-0.3ug/ml, observed in human liver lysates on ~28kD bandsImmunohistochemistry: 3.75ug/ml on paraffin embedded human tonsilOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human DPM1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to RELEVRSPRQNKYS-C, from human DPM1, at N-terminal (NP_003850.1).
