Part of an E3 ubiquitin ligase complex for neddylation.
Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation.
Functions to recruit the NEDD8-charged E2 enzyme to the cullin component.
Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity.
Acts also as an oncogene facilitating malignant transformation and carcinogenic progression.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16.000Western Blot: 0.3-1ug/ml, observed in HepG2 lysates on ~28kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human DCUN1D1. Species sequence homology: Bovine and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mo
Immunogen：Synthetic peptide corresponding to C-RPQIAGTKSTT, from human DCUN1D1, at C-terminal (NP_065691.2).
