Transcription factor that is involved in regulation of organogenesis.
Seems to be a regulator of SIX1, SIX6 and probably SIX5.
Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex.
Transcriptional activation seems also to involve association of CREBBP.
Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter.
Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1.
Binds to chromatin DNA via its DACHbox-N domain.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.1-0.3ug/ml, observed in human kidney lysates on ~75, 52kD bandOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human DACH!. Species sequence homology: Canine, mouse, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to SPVENTPQNNECK, from human DACH1, at the internal region of the protein (NP_542937.1, NP_542938.1, NP_004383.2).
