The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91kD glycoprotein (heavy chain) and a 22kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense.
NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections.
Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response.
CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD).
In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.
The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000Western Blot: 0.03-0.1ug/ml, observed in human peripheral blood mononucleocytes lysates on ~85kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human CYBB.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-SYLNFARKRIKNP, from human CYBB, at the internal region of the protein (NP_000388.2). Species sequence homology: canine
