CUL-2 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5), homologous to the S.
cerevisiae cdc53 gene.
CUL-1 forms a complex with human p19skp1 and F box protein p45skp2.
Together with cdc53 (known as CulA), the three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins.
In contrast to CUL-1, CUL-2 does not associate with p19skp1 and p45skp2.
Instead, CUL-2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL.
The function of this association remains unknown.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 1-3ug/ml, lysates of cell line NIH3T3 observed on ~100kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes mouse CUL2. Species sequence homology: Bovine, canine, human and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-QYIERSQASADE, from mouse CUL2, at C-terminal (NP_003582.2).
