Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins.
Prevents stress-induced aggregation of blood plasma proteins.
Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro).
Does not require ATP.
Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70.
Does not refold proteins by itself.
Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation.
Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement.
Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins.
Promotes proteasomal degradation of COMMD1 and IKBKB.
Modulates NF-kappa-B transcriptional activity.
Nuclear isoforms promote apoptosis.
Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis.
Plays a role in the regulation of cell proliferation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.3-1ug/ml, observed in human colon, kidney and prostate lysates on ~35-37kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human CLU.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-EKALQEYRKKHREE, from human CLU, at C-terminal (NP_001822.2, NP_976084.1). Species sequence homology: canine
