CD80 (B7-1) is a 60kD cell surface glycoprotein which is a member of the CD28/B7 family.
In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4.
CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells.
CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation.
The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Western Blot: 0.3-1ug/ml, observed in human lymph node and tonsil lysates on ~60kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human CD80.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-HLRVNQTFNWNTTK, from human CD80, at the internal region of the protein (NP_005182.1).
