This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane.
The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml, rat retina lysates and 50kDa in mouse and rat kidney lysates observed on ~48kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes ATP1B1 Species Crossreactivity: Mouse and rat. Species sequence homology: Human, bovine, canine and porcine.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Bo Ca Hu Mo Po Rt
Immunogen:Synthetic peptide corresponding to C-KTEISFRPNDPKSYE, from ATP1B1, at the internal region of the protein (NP_001668.1, NP_001001787.1).