Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain.
ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK.
ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins.
Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway.
It may also be involved in hormone-specific desensitization of TSH receptors.
ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells.
It is widely expressed in most of the tissues, especially neuronal tissues and spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Immunohistochemistry (Paraffin): 4-6ug/mlWestern Blot: 0.3-1ug/ml, observed in human brain lysates on ~40kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human ARRB2. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-HDHIPLPRPQS, from ARRB2, at the internal region of the protein (NP_004304.1, NP_945355.1).
