ARAP1 is a 1450aa containing signaling protein, belonging to the centaurin family with SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains.
In vitro, ARAP1 displays RHO-GAP and phosphatidylinositol (3, 4, 5) trisphosphate (PIP3)-dependent ARF-GAP activity that regulates ARF-, RHO-, and CDC42-dependent cell activities.
It associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia.
It is thus involved in both signal transduction and cellular trafficking transport of a receptor protein involved in apoptosis.
This protein is likely involved in the regulation of the cell-specific trafficking of TNFRSF10A and might thus affect the efficacy of TRAIL-induced apoptosis.
ARAP1, an adaptor protein, is ubiquitously expressed in most of the tissues.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes mouse ARAP1. Species sequence homology: Bovine, canine, human, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-AFTADPLSLLRNV, from mouse ARAP1, at C-terminal (NP_056057.2, NP_001035207.1, NP_001128662.1).
