Apolipoprotein F (Apo F), also known as lipid transfer inhibitor protein (LTIP) based on its ability to inhibit lipid transfer between lipoproteins, is a protein component of several lipoprotein classes and a physiologic regulator of cholesteryl ester transfer protein (CETP) function.
Apo F activity was reported to be localized to LDL, consistent with its greater inhibitory activity on this lipoprotein.
Apo F activity is controlled by its reversible incorporation into an inactive complex which provides for short-term fine-tuning of lipoprotein remodeling mediated by CETP.
ApoF overexpression reduces HDL cholesterol levels by increasing clearance of HDL-CE.ApoF may be an important determinant of HDL metabolism and reverse cholesterol transport.
Applications:Suitable for use in Peptide ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Peptide ELISA: 1:32,000Immunohistochemistry (Paraffin): 2.5-3.8ug/mlWestern Blot: 0.3-1ug/ml, observed in human liver lysates on ~35kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human APOF.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KSYDLDPGAGSLE at the C-terminal of human APOF (NP_001629.1).
