APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein).
The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist.
APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
APH1 is widely expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.05-0.1ug/ml, observed in human brain, cerebral cortex, lysates on ~26kD bandsImmunohistochemistry: 5ug/ml on paraffin embedded Human Placenta and Kidney
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human APH1A. Expected to recognize isoform 1 (NP_001071096.1) and isoform 2 (NP_057106.2).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-HVTDRSDARLQYG, at the internal region of human APH1A. Species sequence homology: Bovine, canine, mouse, porcine and rat.
