The AIRE gene function is not well defined, however the protein does contain zinc finger motifs suggestive of a transcription factor.
The protein (isoform 1) is localized to both the nucleus and cytoplasm.
Three splice variant mRNAs products have been described [1].
The longer AIRE-1 mRNA appears to be more abundant and includes exons 1 through 14.
Splice variant AIRE-2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14.
Variant AIRE-3 includes the same exon 1-8-9 sequences as found in AIRE-2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized.
The resulting protein products of these splice variants are differ significantly.
Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.3-1ug/ml, observed in human spleen lysates on ~55kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human AIRE. Species Crossreactivity: Mouse. Species sequence homology: Bovine, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-KAKPPKKPESSAEQ, from AIRE, at the internal region of the protein (NP_000374.1, NP_000649.1).
