ADAM12 is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases.
It is synthesized as a 909aa preproprecursor that contains a 25kD proregion (aa29-207) and a 92kD mature molecule.
Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression.
A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205aa (705-909).
This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator.
Over aa29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.1-0.3ug/ml, observed in human heart lysates on ~90kD bandsImmunohistochemistry (Paraffin): 3-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human ADAM12.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to AARPLPVSPARALC, from human ADAM12, at N-terminal (NP_003465.3, NP_067673.2).
