Aconitase 2 is an enzyme of the TCA cycle and is encoded in the nucleus and active within the mitochondrion.
Aconitase catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle.
Isoforms of ACO2 have been reported but are not well characterized.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.01-0.03ug/ml, observed in human heart lysates on ~90kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human Aconitase 2. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-QDTYQHPPKDSSGQH, from human Aconitase 2, at the internal region of the protein (NP_001089.1).
