After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1-3ug/ml detects a band of ~55kD in human brain (cerebellum, hippocampus, frontal cortex) lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes human CHRNB1.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Bo Hu Mo Rt
Immunogen:Synthetic peptide corresponding to C-QEQEDHDALKED, from an internal region of human CHRNB1 (NP_000738.2). Species sequence homology: bovine, mouse, rat