The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways.
14-3-3 proteins are highly conserved and ubiquitously expressed.
There are at least seven isoforms that have been identified in mammals.
The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes.
This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
Recombinant human YWHAQ was expressed in E.
coli and purified by using conventional chromatography techniques.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Western Blot: 0.03-0.1ug/ml, observed in human brain (hippocampus) lysates on ~28kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human YWHAQ.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-DDRKQTIDNSQ, from human YWHAQ, at the internal region of the protein (NP_006817.1). Species sequence homology: Canine, mouse and rat.
